An ATP pool associated with adenyl cyclase of brain tissue |
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Authors: | H. Shimizu H. Okayama |
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Affiliation: | Kumamoto University Medical School, First Department of Pharmacology, Honjo, Kumamoto, Japan 860 |
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Abstract: | R adioactive adenine has been frequently used to label cyclic adenosine 3', 5'-monophosphate (cAMP) in diverse cells and tissues (K uo and D e R enzo , 1969; S himizu , D aly and C reveling , 1969, B rooker , 1971). We have previously reported that cAMP from the guinea-pig cerebral cortex is derived from a precursor pool of adenine nucleotides which is more highly labelled than the bulk cellular ATP (S himizu , C reveling and D aly , 1970a). The present study was carried out to throw additional light on the property of the precursor pool. Levels of cAMP and ATP and specific activities of [14C]cAMP and [14C]ATP were determined simultaneously in cerebral slices which were prelabelled with [14C]adenine and subsequently incubated with veratridine, histamine or adenosine. |
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