|
|||||
|
|
Detection of a CpA methylase in an insect system: characterization and substrate specificity |
|
| Authors: | Chalasani Devajyothi Vani Brahmachari |
| Affiliation: | (1) Department of Microbiology and Cell Biology, Indian Institute of Science, 560 012 Bangalore, India;(2) ICMR Centre for Genetics and Cell Biology, Indian Institute of Science, 560 012 Bangalore, India;(3) Present address: Department of Molecular Biology, NN1-26, The Cleveland Clinic, Foundation, 9500, Euclid Avenue, OH-44195, USA |
| Abstract: | A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependance similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates. |
| Keywords: | DNA methylation coccids processive enzyme RNA inhibition CpI methylation |
| 本文献已被 SpringerLink 等数据库收录！ | |