Mating success of timed pregnancies in Sprague Dawley rats: Considerations for execution |
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| Institution: | 1. Dept of Basic Medical Science, Faculty of Health Sciences, Durban University of Technology, Durban, South Africa;2. School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa;3. Biomedical Resource Unit, University of KwaZulu-Natal, Westville Campus, Durban, South Africa;4. Optics and Imaging Centre, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, South Africa;1. Department of Dentistry and Oral Surgery, Fukushima Medical University, School of Medicine, Fukushima, Japan;2. Department of Dentistry and Oral Surgery, Tsuruoka Municipal Shonai Hospital, Tsuruoka, Japan;3. Department of Dentistry, Kashima Hospital, Ibaraki, Japan;4. Department of Head and Neck, Facial Surgery, Ohara General Hospital, Fukushima, Japan;1. Department of Obstetrics and Gynecology, Clinical Medical College, Yangzhou University, Yangzhou 225001, China;2. State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China;1. Pelvic Floor and Postpartum Rehabilitation Center, Changsha Maternal and Child Health Care Hospital, Changsha, Hunan 410007, PR China;2. Department of Gynaecology, Changsha Maternal and Child Health Care Hospital, Changsha, Hunan 410007, PR China;1. Centro de Microscopia Eletronica, Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Campus Soane Nazare de Andrade, 45662-900, Ilheus, Brazil;2. Departamento de Fisiologia e Biofísica, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, Brazil;3. Departamento de Clinica e Cirurgia Veterinarias, Escola de Veterinaria, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, Brazil;1. Departament of Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Humanities and Exact Sciences, São José do Rio Preto, São Paulo, Brazil;2. Institute of Biomedical Sciences. Federal University of Uberlandia, Uberlândia, Minas Gerais, Brazil;3. Department of Structural and Functional Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil |
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| Abstract: | This study compares three different mating techniques in Sprague-Dawley rats, using the pregnancy rate as the main indicator of success. It provides recommendations for timed-pregnancy experiments to achieve an appropriate sample size for the study of human pregnancy disorders. The implementation of a preconditioning phase, determination of the estrous cycle, the use of two mating strategies (Lee-Boot and Whitten effect), female: male mating ratios, and cohabitation duration should be considered as they improve the mating success rate. |
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| Keywords: | Mating Estrous cycles Timed pregnancies Success rate Sprague Dawley rats |
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