细胞质雄性不育水稻的呼吸作用和自由基代谢的关系初探

用呼吸电子传递细胞色素途径的抑制剂氰化钾（ＫＣＮ）与抗氰呼吸途径的抑制剂水杨基氧肟酸（ＳＨＡＭ）处理水稻细胞质雄性不育系（ＣＭＳ）珍汕９７Ａ及其保持系珍汕９７Ｂ的幼穗和花药后，ＫＣＮ使不育系与保持系的超氧阴离子自由基（Ｏ２■）产生受到抑制，不育系的Ｏ２■的形成受抑制较多。ＳＨＡＭ处理则增高Ｏ２■形成，以不育系的增加较多．ＫＣＮ与ＳＨＡＭ处理后都使不育系与保持系的丙二醛（ＭＤＡ）含量升高，ＫＣＮ使保持系的ＭＤＡ含量升高较多，ＳＨＡＭ则使不育系的ＭＤＡ含量升高较多．ＫＣＮ处理后，不育系与保持系的超氧物歧化酶（ＳＯＤ）活性下降，ＳＨＡＭ处理后不育系与保持系的ＳＯＤ活性变化不明显。Ｈ２Ｏ２处理对不育系与保持系幼穗的呼吸速率影响不大．Ｈ２Ｏ２＋ＦｅＳＯ４处理后，使呼吸速率大幅度下降，表明Ｈ２Ｏ２＋ＦｅＳＯ４所形成的羟自由基（ＯＨ）比Ｈ２Ｏ２对呼吸代谢的破坏作用更大。

THE RELATIONSHIP BETWEEN RESPIRATION AND ACTIVATED OXYGEN METABOLISM IN CYTOPLASMIC MALE STERILE RICE

The superoxide radical (O2) formation rates in the young panicles and anthers ofcytoplasmic male sterile (CMS) rice Zhenshan 97A (97A) and its maintainer Zhenshan 97B(97B) were inhibited by KCN, and accelerated by salicylhydroxamic acid (SHAM), but thepercentage of O2 formation inhibited by KCN or accelerated by SHAM in sterile rice 97Awas more than that in fertile rice 97B. The malondialdehyde (MDA) content in both of97A and 97B increased after KCN or SHAM treatment, but the great change of MDA wasfound in 97B treated by KCN or in 97A treated by SHAM. Superoxide dismutase (SOD)activity was inhibited by KCN, but was not affected by SHAM. The respiratory rates of97A and 97B decreased after H2O2 FeSO4 treatment while it did not change by H2O2treatment alone.