Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads |
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Authors: | Sanjay Kar Asish Mandal Pradeep K Das Mohapatra Saptadip Samanta Bikash R Pati Keshab C Mondal |
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Institution: | (1) Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India |
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Abstract: | In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous
fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200,
300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase
significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml−1 at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth
cycle (3.36 ± 0.2 U ml−1). |
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Keywords: | Xylanase Immobilization Trichoderma reesei |
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