Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity |
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| Authors: | Abu ZM Saleh Kunitoshi Yamanaka Hironori Niki Teru Ogura Mitsuyoshi Yamazoe Sola Hiraga |
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| Institution: | Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kuhonji 4-24-1, Kumamoto 862, Japan |
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| Abstract: | Abstract The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013 , causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP binding activity remained unaffected. A truncated MukB protein that is short of 109 amino acids from the C-terminus failed to bind DNA. |
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| Keywords: | Escherichia coli MukB protein DNA binding protein Nucleotide binding activity Chromosome partition |
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