| 环介导等温扩增技术对解脲脲原体的临床检测 |
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| 引用本文： | 王毅超,谢姣贵,吴波,张春玲,李招云.环介导等温扩增技术对解脲脲原体的临床检测[J].中国微生态学杂志,2020,32(1):83-86. |
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| 作者姓名： | 王毅超 谢姣贵 吴波 张春玲 李招云 |
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| 作者单位： | 台州市中心医院台州学院附属医院,浙江 台州 318000,解放军九四八医院 泌尿外科,新疆 乌苏 833000,台州市中心医院台州学院附属医院,浙江 台州 318000,台州市中心医院台州学院附属医院,浙江 台州 318000,台州市中心医院台州学院附属医院,浙江 台州 318000 |
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| 基金项目： | 浙江省医药卫生科技项目(2019KY788) |
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| 摘 要： | 目的建立并优化环介导等温扩增(LAMP)技术对解脲脲原体(U.urealyticum)的检测,并应用于临床样本分析。方法针对U.urealyticum的urease基因设计LAMP引物;研究LAMP的最适温度、最佳检测时间及灵敏度和特异度;与传统PCR检测进行方法学比对。结果 LAMP技术检测U.urealyticum的最适温度和最佳时间分别是61℃和60 min,并且具有良好灵敏度和特异度,较普通PCR检测的灵敏度高出1 000倍。临床样本检测中,PCR和LAMP技术达到的灵敏度分别为25.00%和87.50%。两种方法的特异度均为100.00%。结论 LAMP与PCR相比在基层检测和大规模筛查方面有显著的优势和巨大的利用价值。
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| 关 键 词： | 恒温扩增技术 解脲脲原体 泌尿生殖道支原体 |
| 收稿时间： | 2018/12/21 0:00:00 |
| 修稿时间： | 2019/4/30 0:00:00 |
The clinical detection of Ureaplasma urealyticum with loop-mediated isothermal amplification |
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| WANG Yichao,XIE Jiaogui,WU Bo,ZHANG Chunling and LI Zhaoyun.The clinical detection of Ureaplasma urealyticum with loop-mediated isothermal amplification[J].Chinese Journal of Microecology,2020,32(1):83-86. |
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| Authors: | WANG Yichao XIE Jiaogui WU Bo ZHANG Chunling and LI Zhaoyun |
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| Institution: | (Taizhou University Hospital) |
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| Abstract: | Objective To establish and optimize loop-mediated isothermal amplification(LAMP) assay for detection of Ureaplasma urealyticum(U. urealyticum) in clinical samples. Methods LAMP primers were designed to specifically target urease of U. urealyticum. The specificity and sensitivity of LAMP were explored. Results The sensitivity of LAMP was found to be 100 pg for U. urealyticum, which was higher than that of conventional PCR. Conclusion The LAMP assay can potentially facilitate simultaneous detection of mycoplasma in a large number of samples in clinical practice, which can be used as a primary screening method and as a supplementary method for classical methods. |
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| Keywords: | Loop-mediated isothermal amplification Ureaplasma urealyticum Genitourinary mycoplasma |
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