Rapid screening of <Emphasis Type="Italic">Salmonella enterica</Emphasis>serovars Enteritidis,Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles |
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| Authors: | Yang Hong Tongrui Liu Margie D Lee Charles L Hofacre Marie Maier David G White Sherry Ayers Lihua Wang Roy Berghaus John J Maurer |
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| Institution: | (1) Department of Population Health, The University of Georgia, Athens, GA 30602, USA;(2) Department of Infectious Diseases, The University of Georgia, Athens, GA, USA;(3) Department of Statistics, The University of Georgia, Athens, GA 30602, USA;(4) Center for Food Safety and Quality Enhancement, The University of Georgia, Griffin, GA 30223, USA;(5) Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA;(6) USDA ARS, Russell Research Center, 950 College Station road, Athens, GA 30605, USA;(7) T. Liu- Emory University, 1701 Uppergate Drive, Atlanta, GA 30322, USA |
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| Abstract: | Background Classical Salmonella serotyping is an expensive and time consuming process that requires implementing a battery of O and H antisera to detect
2,541 different Salmonella enterica serovars. For these reasons, we developed a rapid multiplex polymerase chain reaction (PCR)-based typing scheme to screen
for the prevalent S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. |
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