| 用核酸免疫技术制备抗丙肝病毒C区单克隆抗体 |
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| 引用本文： | 李益民 周旭. 用核酸免疫技术制备抗丙肝病毒C区单克隆抗体[J]. 微生物学免疫学进展, 1998, 26(1): 64-65 |
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| 作者姓名： | 李益民 周旭 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 用丙肝病毒Ｃ＋Ｅ１区真核表达质粒ｐｃＤＮＡ－ＨＣＶ／Ｃ＋Ｅ１按４００ｕｇ／只剂量免疫ＢＡＬＢ／Ｃ小鼠，１４周后同一剂量再加强免疫一次。加强免疫后２周，在５０％ＰＥＧ１４５０介导下将脾细胞与ＳＰ２／０小鼠骨髓瘤细胞（５１）融合。实验结果融合率达５４．３％（３１３／５７６）。阳性率为５．４％（１７”３１３）。克隆化后得到６株稳定分泌抗丙肝病毒Ｃ区单克隆抗体杂交瘤细胞株。这６株杂交瘤均产生ＩｇＭ抗体，接种ＢＡＬＢ／Ｃ小鼠后产生腹水的效价为１６４－１３２０（ＥＬＩＳＡ）。
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| 关 键 词： | 丙肝病毒 核酸免疫 单克隆抗体 杂交瘤 |
Preparation of Monoclonal Antibodies against Hepatitis C/C by Immunizing Mice with DNA |
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| Abstract: | The BALB/C mouse was immunized with 400 ug hepatitis C virus/C+E1 eukaryotic expression plasmid-pcDNA HCV/C+E1.After 14 weeks,the mouse was boosted with the same amount of pcNDA-HCV/C+E1 plasmid.On the 14th day after last immunization,the fusion of the spleen cells and SP2/0 myloma cells (5∶1) was carred out.The fusion produced 313 wells of hybridoma.Of these growing wells,5.4% (17/313) contained antibody against gene-recombinant HCV/C antigen. After clonning,6 strains of hybridoma were obtained. All of these produced IgM to HCV/C. The titers of the antibodies in ascites were 1∶64 to 1∶320 (ELISA). |
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| Keywords: | hepatitis C DNA immunization monoclonal antibody Hybridoma |
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