Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase |
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Authors: | Nazir Basiran Philip Armitage Roderick John Scott John Draper |
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Institution: | (1) Department of Botany, University of Leicester, University Road, LE1 7RH Leicester, UK |
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Abstract: | Genetic transformation of flax (Linum usitatissimum) has been achieved using an A. tumefaciens strain carrying a non-oncogenic Ti plasmid-derived vector containing a chimaeric npt-II gene and a wild type nopaline synthase gene. Fertile, transformed shoots were most easily obtained from Kmr callus developing on hypocotyl sections. The totipotency of the Kmr callus was dependent upon its origin. T-DNA was visualised by Southern blotting in all Kmr tissues. Efficient expression of nopaline synthase and the chimaeric npt-II gene was found in transformed Kmr callus and regenerated shoots.Abbreviations
npt-II
neomycin phosphotransferase II gene
- NPT-II
neomycin phosphotransferase II
- nos
nopaline synthase gene promoter
- Kmr
kanamycin resistant
- BAP
6-benzylaminopurine
- NAA
-naphthaleneacetic acid
- MSD4×2
medium D4×2 based on Murashige & Skoog medium (see Scott & Draper, 1987) |
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