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Time course analysis of RNA stability in human placenta |
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| Authors: | Isabelle Fajardy Emmanuelle Moitrot Anne Vambergue Maryse Vandersippe-Millot Philippe Deruelle Jean Rousseaux |
| Affiliation: | 1. Centre de Biologie Pathologie, P?le de Biochimie et Biologie Moléculaire, CHRU de Lille, Université, Lille 2, France 2. Service de Diabétologie et d'Endocrinologie, CHRU de Lille, France 3. Service de Gynécologie Obstétrique, CHRU de Lille, France |
| Abstract: | BackgroundEvaluation of RNA quality is essential for gene expression analysis, as the presence of degraded samples may influence the interpretation of expression levels. Particularly, qRT-PCR data can be affected by RNA integrity and stability. To explore systematically how RNA quality affects qRT-PCR assay performance, a set of human placenta RNA samples was generated by two protocols handlings of fresh tissue over a progressive time course of 4 days. Protocol A consists of a direct transfer of tissue into RNA-stabilizing solution (RNAlater™) solution. Protocol B uses a dissection of placenta villosities before bio banking. We tested and compared RNA yields, total RNA integrity, mRNA integrity and stability in these two protocols according to the duration of storage. |
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