Microsatellite tagging of the stripe-rust resistance gene YrH52 derived from wild emmer wheat, Triticum dicoccoides, and suggestive negative crossover interference on chromosome 1B |
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Authors: | J H Peng T Fahima M S Röder Y C Li A Dahan A Grama Y I Ronin A B Korol E Nevo |
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Institution: | (1) Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel Fax: +972-4-8246554 E-mail: nevo@research.haifa.ac.il, IL;(2) Institute for Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466 Gatersleben, Germany, DE;(3) Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel, IL |
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Abstract: | Stripe rust caused by Puccinia striifomis West. is one of the most devastating diseases relating to wheat production. Wild emmer wheat, Triticum dicoccoides, the tetraploid progenitor of cultivated wheat, has proven to be a valuable source of novel stripe-rust resistance genes
for wheat breeding. For example, T. dicoccoides accessions from Mt. Hermon, Israel, are uniformly and highly resistant to stripe-rust. The main objective of the present
study is to map a stripe-rust resistance gene, derived from the unique Mt. Hermon population of wild emmer, using microsatellite
markers. An F2 mapping population was established by crossing stripe-rust resistant T. dicoccoides accession H52 from Mt. Hermon with the Triticum durum cultivar Langdon. The stripe-rust resistance derived from accession H52 was found to be controlled by a single dominant gene
which was temporarily designated as YrH52. Out of 120 microsatellite markers tested, 109 (91%) showed polymorphism between the parental lines. Among 79 segregating
microsatellite loci generated from 56 microsatellite primer pairs, nine were linked to YrH52 with recombination frequencies of 0.02–0.35, and LOD scores of 3.56–54.22. A genetic map of chromosome 1B, consisting of
ten microsatellite loci and the stripe-rust resistance gene YrH52, was constructed with a total map length of 101.5 cM. YrH52 is also closely linked to RFLP marker Nor1 with a map distance of 1.4 cM and a LOD value of 29.62. Apparent negative crossover interference was observed in chromosome
1B, especially in the region spanning the centromere. Negative crossover interference may be a common characteristic of gene-rich
regions or gene clusters in specific chromosomes.
Received: 30 October 1998 / Accepted: 2 November 1998 |
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Keywords: | Genetic mapping Microsatellite marker Stripe-rust resistance Puccinia striiformis Negative crossover interference Triticum dicoccoides Wild emmer wheat |
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