Affinity enhancement of bispecific antibody against two different epitopes in the same antigen. |
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Authors: | H S Cheong J S Chang J M Park S M Byun |
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Institution: | Immunology Laboratory, Mogam Biotechnology Research Institute, Kyonggi-Do, Korea. |
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Abstract: | For the enhancement of antibody binding affinity, a bispecific antibody against two different epitopes in human chorionic gonadotropin hormone, one is in alpha-subunit and the other is in beta-subunit, was prepared by chemical recombination using 5,5'-dithiobis(2-nitrobenzoic acid). The epitopes recognized by antibodies were investigated by competitive radioimmunoassay, two-site sandwich radioimmunoassay and additivity assay and a proper epitope pair was chosen for preparation of the bispecific antibody. This bispecific antibody has dual specificity and as much as 17.2-fold higher affinity than that of monoclonal antibody with higher affinity by dual antigen binding radioimmunoassay and Scatchard plot analysis. |
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