聚合酶链反应-单链构型多态性检测细胞壁缺陷型淋病奈瑟菌cppB基因变异

目的：检测与分析淋病奈瑟菌L型的cppB基因，探讨细胞壁缺陷对淋病奈瑟菌cppB基因的影响。方法：用青霉素诱导淋病奈瑟菌成为L型并获得稳定L型纯培养物，用cppB基因特异性引物以聚合酶链反应(PCR)检测稳定L型纯培养物的cppB基因和进行单链构型多态性(SSCP)分析。结果：淋病奈瑟菌的细菌型及其L型都具有cppB基因扩增产物，但PCR—SSCP分析可见异常泳动DNA带型(细菌型有2条带、L型有3条带)。结论：细胞壁缺陷淋病奈瑟菌仍然具有cppB基因，但其碱基序列可以发生改变。

DETECTION THE CRYPTIC PLASMID B GENE VARIATION IN NEISSERIA GONORRHOEAE L-FORMS BY PCR-SINGLE STRAND CONFORMATION POLYMORPHISM

Objective:To detect the cppB gene in L - forms of Neisseria gonorrhoeae and probe the influence of cell wall deficien cy on the Cryptic Plasmid B gene of N gonorrhoeae Methods:The N gonorrhoeae was induced into L-forms by p enicillin and the cppB gene of the pure culture of L-form was determined by PCR and SSCP.Results:By PCR,the cppB genes would be found in both the bacter ial form and the L-forms of N gonorrhoeae There were two bands in the ba cterial form and three bands in L-forms by PCR-SSCP.Conclusion:The cppB gene is still contained in the cell wall de ficient N gonorrhoeae but its base alignment would be changed.