Cytochemical localization of vanadium(III) in blood cells of ascidian Phallusia mammillata Cuvier,and its relevance to hematic cell lineage determination |
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| Institution: | 1. Ruđer Bošković Institute, Center for Marine Research, G. Paliage 5, 52210 Rovinj, Croatia;2. Institute of Oceanography and Fisheries, Šetalište I. Meštrovića 63, 21000 Split, Croatia;3. Istituto Nazionale di Oceanografia e Geofisica Sperimentale, Oceanography section, via Auguste Piccard 54, 34151 Trieste, Italy;4. National Research Council, Institute of Marine Sciences, Arsenale Tesa 104 Castello 2737/f, 30122 Venice, Italy;5. National Research Council, Institute of Marine Sciences, Largo Fiera della Pesca 2, 60125 Ancona, Italy;6. Agricultural University of Tirana, Kodër Kamëz, SH1, 1000 Tirana, Albania;7. University of Dubrovnik, Institute for Marine and Coastal Research, Kneza Damjana Jude 12, 20000 Dubrovnik, Croatia;8. University of Montenegro, Institute of Marine Biology, Dobrota bb, 85330 Kotor, Montenegro;9. National Institute of Biology, Marine Biology Station, Fornače 41, 6330 Piran, Slovenia;10. Ruđer Bošković Institute, Division for Marine and Environmental Research, Bijenička 54, 10000 Zagreb, Croatia;11. The University of Western Australia, School of Civil, Environmental and Mining Engineering & UWA Oceans Institute, Crawley, WA 6009, Australia;12. Institute for Environmental Protection and Research, Via Vitaliano Brancati 48, 00144 Rome, Italy;13. University of Dubrovnik, Department of Aquaculture, Ćira Carića 4, 20000 Dubrovnik, Croatia;1. KMU - Kaohsiung Medical University, Dept. of Biomedical Science and Environmental Biology, No. 100, Shin-Chuan 1st Road, KAOHSIUNG 80708, Taiwan R.O.C.;2. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung 80424, Taiwan, R.O.C.;3. Institute of Marine Biology, National Taiwan Ocean University, Keelung, 20224 Taiwan R.O.C. |
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| Abstract: | When the blood cells of ascidians Phallusia mammillata are stained with the ligand 2,2′-bipyridine, those cells which contain vanadium(III), in an easily sequestered form, take up the stain producing in situ, a purple complex. This material extracted displays spectral characteristics consistent with the formation of an oxo-bridge vanadium(III) bipyridine dimer. The staining is localized in the signet ring cell, a bivacuolated cell, a cell type with numerous darkly staining compartments, and also by the vacuolated amoebocyte. The possible ramifications of these observation are discussed in relation to the delineation of the signet ring cell lineage. |
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