| 重组痘病毒表达SARS冠状病毒纤突蛋白及其免疫原性分析 |
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| 引用本文： | 胡森,王清华,王喜军,王笑梅,步志高.重组痘病毒表达SARS冠状病毒纤突蛋白及其免疫原性分析[J].病毒学报,2007,23(4):287-291. |
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| 作者姓名： | 胡森 王清华 王喜军 王笑梅 步志高 |
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| 作者单位： | 1. 中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨,150001
2. 农业部动物检疫所,国家外来动物疫病诊断中心,青岛,266032 |
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| 基金项目： | 国家科技攻关计划，国家重点基础研究发展计划(973计划) |
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| 摘 要： | 对牛痘病毒弱毒株表达的SARS冠状病毒纤突蛋白(Spike protein,S)的免疫原性进行分析与比较.以减毒痘病毒(WR株)为载体重组了SARS冠状病毒全长S基因(rWR-SARS-S).SDS-PAGE和Western blot试验表明,迁移率约为190kD的重组SARS S蛋白可在HeLa细胞中表达,而且可以被鸡抗SARS全病毒高免血清识别,具有特异免疫反应原性.进一步研究表明,rWR-SARS-S感染的细胞在IFA试验中可与鸡抗SARS的高免血清发生特异反应,具有良好的敏感性和特异性.以104PFU的rWR-SARS-S免疫BALB/c小鼠产生的抗体在间接ELISA试验中可以被S蛋白识别,产生特异抗原抗体反应.利用痘病毒表达的SARS冠状病毒S蛋白具有良好的抗原性和生物学活性,可替代SARS冠状病毒全病毒,为研究安全、敏感和特异的重组诊断抗原奠定了重要基础.
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| 关 键 词： | SARS冠状病毒 纤突蛋白 重组痘病毒 |
| 文章编号： | 1000-8721（2007）04-0287-05 |
| 修稿时间： | 2006-11-212007-03-09 |
Expression of Recombinant Spike Protein of SARS-Coronavirus in Vaccinia Virus and Analysis of Its Immunogenicity |
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| HU Sen,WANG Qing-hua,WANG Xi-jun,WANG Xiao-mei,BU Zhi-gao.Expression of Recombinant Spike Protein of SARS-Coronavirus in Vaccinia Virus and Analysis of Its Immunogenicity[J].Chinese Journal of Virology,2007,23(4):287-291. |
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| Authors: | HU Sen WANG Qing-hua WANG Xi-jun WANG Xiao-mei BU Zhi-gao |
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| Institution: | 1. Harbin Veterinary Research Institute, Chinese Academy of Agriculture Science, Harbin 150001, China;2.National Diagnostic Center for Exotic Animal Disease, National Animal Quarantine Institute of Ministry of Agriculture, Qingdao 266032, China |
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| Abstract: | A recombinant vaccinia virus(rWR-SARS-S)expressing spike protein of severe acute respiratory syndrome coronavirus was constructed.The expression of full length recombinant SARS spike protein(rSS) in HeLa cells possessing specific reaction ability to chicken anti-sera was confirmed by SDS-PAGE and Western-blot(190kD).HeLa cells infected with rWR-SARS-S also showed high sensitivity in detecting specific serum antibody by indirect immunofluoresence assay(IFA).The results above indicated that the availability of such a faithful model system offers particular advantages for the study of SARS in that it reduces the need for direct manipulation of an exotic pathogen.In the absence of infectious SARS,we may safely carry out detailed biochemical and genetic manipulations to investigate features of viral replication and gene function,as well as explore new avenue for vaccine development. |
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| Keywords: | IFA |
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