DNA probes for the Y-chromosome ofSilene latifolia,a dioecious angiosperm |
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| Authors: | D L Mulcahy N F Weeden R Kesseli S B Carroll |
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| Institution: | (1) Department of Botany, University of Massachusetts, 01003 Amherst, MA, USA;(2) Department of Horticultural Sciences, Cornell University, 14456 Geneva, NY, USA;(3) Department of Vegetable Crops, University of California, 95616 Davis, CA, USA;(4) Department of Biology, Indiana University, 47405 Bloomington, IN, USA |
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| Abstract: | Summary In order to obtain markers for the Y chromosome ofSilene latifolia, we pooled equal weights of leaf tissue from 18 female siblings into one sample and repeated the process with 18 male siblings. Pooling was intended to provide a common genetic background for each sample, leaving the absence or presence of the Y chromosome as the primary difference between the two samples. DNA was extracted from each sample and subjected to polymerase chain reaction (PCR) amplification with arbitrary 10 bp primers. Four of 60 primers used gave an amplification with the male DNA not found among those from the female DNA. Each of these was subsequently shown to provide a reliable marker for the Y chromosome. |
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| Keywords: | Sex chromosome markers Y-chromosome Angiosperms Silene latifolia Melandrium album |
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