| 应用SSP-PCR/SSO方法进行中国辽宁汉族人HLA-DRB1基因的遗传多态性研究 |
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| 引用本文： | 刘利民,梁健,宋芳吉,贾静涛.应用SSP-PCR/SSO方法进行中国辽宁汉族人HLA-DRB1基因的遗传多态性研究[J].遗传,1999,21(3):1-24. |
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| 作者姓名： | 刘利民 梁健 宋芳吉 贾静涛 |
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| 作者单位： | 1. 中国医科大学法医血清学教研室
2. 中国医科大学附属第一医院,沈阳,110001 |
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| 基金项目： | 国家教委留学归国人员科研基金,辽宁省科委自然科学基金 |
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| 摘 要： | 对１５９名中国辽宁汉族个体的基因组ＤＮＡ进行分析，共检出４２种等位基因，其中以ＤＲＢ１０９０１２（１２．８％）、０７０１（１０．７％）、１５０１（１０．４％）最为常见，其次为ＤＲＢ１１２０１（７９％）、１２０２（７５％）、１１０１（６６％）、０３０１（５．０％）。并发现辽宁汉族人ＤＲＢ１等位基因频率与白种人间存在明显差异，揭示不同人种有其自己的主要等位基因。同时对本技术在ＨＬＡ－ＤＲＢ１分型应用中的优点进行了讨论
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| 关 键 词： | HLA－DRB1基因分型，SSP－PCR，寡核苷酸探针 |
Polymorphic Analysis of HLA-DRB1 Gene in Chinese Liaoning Han Population by Sequence Specific Primers-PCR and Sequence Specific Oligonucleotide Probes |
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| LIU Li-Min,LIANG Jian,SONG Fang-Ji,JIA Jing-Tao.Polymorphic Analysis of HLA-DRB1 Gene in Chinese Liaoning Han Population by Sequence Specific Primers-PCR and Sequence Specific Oligonucleotide Probes[J].Hereditas,1999,21(3):1-24. |
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| Authors: | LIU Li-Min LIANG Jian SONG Fang-Ji JIA Jing-Tao |
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| Abstract: | HLA- DRB1 alleles of 159 Chinese donors from Liaoning Han population were determined by using a set of 23 sequence specific oligonucleotide probes directed to various DRB1 alleles. The samples were first amplified and divided into 8 groups by allele/group specific primers. The SSOs enable us to identify 58 different DRB1 subtypes. 42 alleles were detected in the study of this population. Among them, the DRB1 *09012(12.8%), *0701(10.7%), *1501(10.4%), *1201(7.9%), *1202(7.5%), *1101(6.6%), *0301(5.0%) are the most frequent. The significant difference was found in Chinese northern Han population by comparing the gene frequencies in Caucasoid samples, suggesting that there were principal DRB1 alleles in different races. |
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| Keywords: | HLA- DRB1 Genotyping SSP-PCR SSO |
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