| 蟾蜍卵母细胞的Na~+/H~+交换 |
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| 引用本文： | 王文辉,李学彬,马青年,程治平.蟾蜍卵母细胞的Na~+/H~+交换[J].动物学报,1986(3). |
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| 作者姓名： | 王文辉 李学彬 马青年 程治平 |
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| 作者单位： | 哈尔滨医科大学生理教研室
(王文辉，李学彬，马青年)，哈尔滨医科大学生理教研室(程治平) |
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| 摘 要： | 应用pH选择性徽电极和普通檄电极测量了蟾蜍卵母细胞内的pH值(pHi)。当用含NH_4~+而不含HCO_3~-的溶液培灌卵母细胞时,pHi逐渐下降,在停止培灌后,pHi恢复到对照值。这个pHi恢复过程是一个H~+的主动转运,用胆碱离子取代培灌液中的Na~+可以抑制pHi的恢复,这种抑制作用是可逆的。伴随着细胞内pH值的恢复,细胞内Na~+活度(Na_1~+)呈现暂时性增加,而细胞内Cl-活度(Cl_1~+)不变或稍有增加。实验结果提示在蟾蜍卵母细胞膜上存在着Na~+/H~+的交换。
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A STUDY ON Na~+/H~+ EXCHANGE OF THE TOAD OOCYTES |
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| WANG WENHUI LI XUEBIN MA QINGNIAN CHENO ZHIPING.A STUDY ON Na~+/H~+ EXCHANGE OF THE TOAD OOCYTES[J].Acta Zoologica Sinica,1986(3). |
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| Authors: | WANG WENHUI LI XUEBIN MA QINGNIAN CHENO ZHIPING |
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| Abstract: | Using conventional and pH-sensitive microeleetrodes to measure intracellular pH (pH1) in isolated oocytes of the toad (Bufo bufo gargarizans), it was found that when oocytes were superfused with NH4+ in a nominally HCO3- -free solution, pH1 was decreased and after withdrawal of NH4+ the pH4 was returned to control value. This pH4 recovery, which indicatied an active transport, failed to occur after removal of Na+ from the superfusion fluid. Ee-addition of Na+ resulted in a full pH4 recovery. A transient rise of intracellular Na+ activity accompanied the pH4 recovery. Intracellular Cl- activity was not changed or slightly increased. These data suggest that the oocytes of toad show Na+/H+ exchange in the membrane. |
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| Keywords: | pH-sensitive microelectrode Intracellular Na+ Cl- pH Na+/H+ exchange Oocyte of toad |
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