| 一株阿特拉津降解菌的分离鉴定及降解特性 |
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| 引用本文： | 闫彩芳,娄旭,洪青,李顺鹏.一株阿特拉津降解菌的分离鉴定及降解特性[J].微生物学通报,2011,38(4):493-497. |
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| 作者姓名： | 闫彩芳 娄旭 洪青 李顺鹏 |
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| 作者单位： | 1. 农业部农业环境微生物工程重点开放实验室,南京农业大学生命科学学院,江苏,南京,210095
2. 农业部农业环境微生物工程重点开放实验室,南京农业大学生命科学学院,江苏,南京,210095;土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,江苏,南京,210008 |
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| 基金项目： | 国家自然科学基金项目(No. 31070099); 土壤与农业可持续发展国家重点实验室开放基金(No. Y052010025) |
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| 摘 要： | 从农药厂废水处理池的活性污泥中分离到一株阿特拉津降解菌X-4, 根据其生理生化特性和16S rRNA基因序列相似性分析, 将其初步鉴定为节杆菌属(Arthrobacter sp.)。该菌能以阿特拉津为唯一碳氮源生长, 42 h内对100 mg/L的阿特拉津降解效果为95.7%, 降解阿特拉津的最适温度为30 °C, pH为7.0。该菌对多种重金属离子都存在抗性, 显示了其在去除阿特拉津和重金属复合污染方面的应用潜力。对其降解基因的初步研究显示, 该菌含有trzN、atzB和atzC 3个阿特拉津降解相关基因。
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| 关 键 词： | 阿特拉津 生物降解 重金属抗性 降解相关基因 |
Isolation, identification and characterization of an atrazine-degrading strain |
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| YAN Cai-Fang,LOU Xu,HONG Qing and LI Shun-Peng.Isolation, identification and characterization of an atrazine-degrading strain[J].Microbiology,2011,38(4):493-497. |
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| Authors: | YAN Cai-Fang LOU Xu HONG Qing and LI Shun-Peng |
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| Institution: | 1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sci-ence, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sci-ence, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sci-ence, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; 2. State Key Laboratory of Soil and Sustainable Ag-riculture,;1. Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sci-ence, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China |
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| Abstract: | A bacteria strain X-4 capable of degrading atrazine was isolated from the activated sludge from the wastewater treating system of an atrazine manufacturer. It was preliminarily identified as Arthrobacter sp. according to its physiological & biochemical characteristics and the analysis of its 16S rRNA gene sequence. This strain could use atrazine as sole carbon and nitrogen source, and degrade 95.7% of 100 mg/L atrazine within 42 h. The optimal pH and temperature for the degradation were 30 °C and pH 7.0, respectively. Strain X-4 could tolerate many kinds of heavy metals, which showed its potential in treating the co-contamination of atrazine and heavy metals. The atrazine-degrading related genes of strain X-4 were the combination of trzN, atzB and atzC. |
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| Keywords: | Atrazine Biodegradation Heavy metal-resistance Degradation related genes |
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