Electrophoresis of proteins and nucleic acids on acrylamide-agarose gels lacking covalent crosslinking |
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| Authors: | P M Horowitz J C Lee G A Williams R F Williams L D Barnes |
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| Institution: | 1. Department of Biochemistry, The University of Texas Health Science Center, San Antonio, Texas 78284 USA;2. Division of Earth and Physical Science, The University of Texas, San Antonio, Texas 78285 USA |
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| Abstract: | The preparation of acrylamide-agarose gels lacking covalent crosslinking with methylenebisacrylamide is described. These hybrid gels melt at 85 degrees C and, consequently, allow quantitative analysis of tritium-labeled protein after electrophoresis. Recovery of tritium-labeled ribonucleic acids extracted from hybrid gels is 20 to 25% greater than from standard acrylamide-methylenebisacrylamide gels. Standard curves of electrophoretic mobilities as a function of molecular weights of dissociated proteins and ribonucleic acids are compared for acrylamide-agarose gels and acrylamide-methylenebisacrylamide gels. |
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| Keywords: | gel electrophoresis proteins nucleic acids agarose acrylamide |
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