| 小麦Rubisco活化酶基因的克隆和表达特性 |
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| 引用本文： | 张国,李滨,邹琦.小麦Rubisco活化酶基因的克隆和表达特性[J].植物学通报,2005,22(3):313-319. |
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| 作者姓名： | 张国 李滨 邹琦 |
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| 作者单位： | 山东农业大学生命科学学院植物系,泰安271018 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Rubisco活化酶是广泛存在于光合生物中调节Rubisco活性的酶,我们利用PCR技术,从小麦(Triticum aestivum)叶片cDNA文库中克隆得到Rubisco活化酶基因cDNA片段,该片段长度为850 bp,编码201个氨基酸.Northern blot表明,小麦叶片在暗诱导衰老的条件下,叶片中活化酶基因表达水平逐渐下降;同时,小麦叶片的光合特性、叶绿素含量和Rubisco活性呈现下降趋势.这些结果表明,衰老时小麦叶片Rubisco活化酶基因表达水平下降与光合速率下降密切相关.
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| 关 键 词： | Rubisco活化酶 小麦 衰老 |
| 修稿时间： | 2004年2月17日 |
Cloning and Expression of Rubisco Activase Gene in Wheat |
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| Abstract: | Rubisco activase is an ubiquitous enzyme for the activation of Rubisco in photosynthetic autotrophs. A cDNA fragment of Rubisco activase gene was cloned from wheat (Triticum aestivum). Northern blot showed that expression of the gene was down-regu- lated in dark-induced senescence leaves, where photosynthetic rate, chlorophyll content and Rubisco activity also showed obvious decline. The results suggest that the decreased expres- sion level of the gene was related to the decline in photosynthetic rate. |
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| Keywords: | Rubisco activase Wheat Senescence |
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