Influence of mechanical activity,adrenergic stimulation,and calcium on the expression of myosin heavy chains in cultivated neonatal cardiomyocytes |
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Authors: | Hans Peter Luther Simone Hille Hannelore Haase Ingo Morano |
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Abstract: | It is generally accepted that mechanical stress of cardiomyocytes increases RNA and protein synthesis of myosin heavy chain (MHC) quantitatively but it is still a matter of debate whether MHC gene expression is also changed qualitatively. We investigated expression of MHC genes of spontaneously contracting neonatal cardiomyocytes experimentally arrested by permanent depolarization potassium chloride (KCI)] as well as by electromechanical uncoupling 2,3 butanedione monoxime (BDM)]. Relative distribution of MHC mRNA isoforms (α and β) was studied by quantitative polymerase chain reaction. Expression of MHC isoenzymes was the same in contracting (34.5% β-MHC) and arrested (40.5% and 33.0% β-MHC in KCl and BDM, respectively) cardiomyocytes. However, treatment with phenylephrine for the same period increased significantly β-MHC expression to 55%. We conclude that hormonal factors rather than Ca2− or mechanical stress regulate qualitatively MHC gene expression. J. Cell. Biochem. 64:458–465. © 1997 Wiley-Liss, Inc. |
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Keywords: | myosin heavy chain gene expression neonatal rat heart culture contraction 2 3 butanedione monoxime |
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