Differences in the expression of lipolytic-related genes in rat white adipose tissues |
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Authors: | Bertile Fabrice Criscuolo François Oudart Hugues Le Maho Yvon Raclot Thierry |
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Institution: | Centre d'Ecologie et Physiologie Energétiques, UPR 9010 CNRS, associé à l'Université Louis Pasteur, 23 rue Becquerel, 67087 2, Strasbourg Cedex, France. Fabarice.Bertile@c-strasbourg.fr |
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Abstract: | We have investigated in vivo whether the gene expression of the beta3-adrenergic receptor (beta3-AR), perilipin A, hormone-sensitive lipase (HSL), and adipocyte lipid-binding protein (ALBP/aP2) is regulated in a site-specific manner. To induce lipolysis and discriminate between short- and long-term modifications, rats were submitted to an experimental fast for one or five days followed or not by refeeding. The mRNA encoding beta3-AR in retroperitoneal adipose tissue (RP) was significantly increased by one and five days of fasting (4-fold) and then lowered by one day of refeeding (2-fold) compared to fed rats. The reverse trend was observed for perilipin A expression in one day fasted rats. HSL mRNA concentrations were significantly induced (2.2-fold) by five days of fasting relative to fed animals and remained high after refeeding. ALBP/aP2, peroxisome proliferator-activated receptor gamma, and CAAT/enhancer binding protein alpha mRNA levels were essentially unaffected by dietary manipulations. Fasting and/or refeeding were similarly ineffective at regulating gene expression in SC. These data provide a molecular basis for regional differences at different steps of the lipolytic process. |
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Keywords: | Lipolysis Body fat distribution Gene expression Fasting/refeeding Time-course changes |
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