Molecular cloning and characterisation of cDNAs complementary to mRNAs from wounded potato (Solanum tuberosum) tuber tissue |
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Authors: | A D Shirras D H Northcote |
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Institution: | (1) Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW Cambridge, UK |
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Abstract: | Five cDNA clones complementary to mRNAs representing different abundances and responses to wounding have been isolated from a library of Sau 3A fragments in the bacteriophage M13 mp8. These were characterised by hybrid-release translation and hybridisation to RNA blots. The levels of RNA complementary to two of the clones show a marked increase during the 24h after wounding, one shows a small increase and two show no appreciable changes except that caused by a general increase in the total amount of polyadenylated RNA per microgram of total RNA which increases 2.5-fold during the same period. The would-induced RNAs are not induced in diluted suspension-culture cells, but RNA complementary to each clone is present in varying levels in stems, leaves and roots of intact potato plants.Abbreviations cDNA
complementary DNA
- poly(A)
polyadenosine
- poly(A)+ RNA
polyadenylated RNA
- poly(U)
polyuridine |
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Keywords: | Bacteriophage (DNA cloning) Complementary DNA (clones) Gene expression Solanum (cDNAs) Wound response |
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