Toxicity of a <Emphasis Type="Italic">Bacillus thuringiensis israelensis</Emphasis>-like strain against <Emphasis Type="Italic">Spodoptera frugiperda</Emphasis> |
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Authors: | Jr" target="_blank">José D Antonino de SouzaJr Sona Jain Claudia Maria Fontes de Oliveira Constância F Ayres Wagner Alexandre Lucena |
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Institution: | 1.Departamento de Zoologia,CCB, UFPE. Av. Prof. Moraes Rego, Cidade Universitária,Recife,Brazil;2.Embrapa Algod?o,Campina Grande,Brazil;3.Departamento de Entomologia,CPqAM, Fiocruz Av. Prof. Moraes Rego, Cidade Universitária,Recife,Brazil |
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Abstract: | Bacillus thuringiensis (Bt) Berliner is a promising agent for microbial control of agriculturally and medically important insects. This study aimed
at searching for Bt strains encoding Cry proteins that act more efficiently against fall armyworm. Thirty Bt strains were
isolated from soil samples in Pernambuco State and evaluated through bioassays. Among these, strain I4A7 was the most efficient
against the fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), and thus it was characterized by biochemical sodium dodecyl sulfate–polyacrylamide
gel electrophoresis (SDS–PAGE) and molecular (polymerase chain reaction (PCR) and sequencing reaction) methods. The protein
pattern of this strain on a SDS–PAGE was similar to that of B. thuringiensis
israelensis (Bti). Moreover, I4A7 cry DNA sequence showed high identity (99–100%) to genes cry4Aa, 4Ba, 10Aa, 11Aa, cyt1Aa and cyt2B from Bti. The toxicity of the newly isolated Bti-like strain upon S. frugiperda should be considered as this strain might be used in combination with other Bt strains, such as B. thuringiensis var. kurstaki (Btk).
Handling Editor: Helen Roy. |
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Keywords: | Bacillus thuringiensis Biochemical characterization δ -Endotoxins Fall armyworm Lepidoptera Noctuidae Molecular characterization |
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