Recombination within mouse <Emphasis Type="Italic">t</Emphasis> haplotypes has replaced significant segments of <Emphasis Type="Italic">t</Emphasis>-specific DNA |
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Authors: | Lyle T Wallace Mark A Erhart |
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Institution: | (1) Department of Biological Sciences, Chicago State University, Chicago, IL 60628, USA;(2) Present address: Wisconsin National Primate Research Center (WNPRC), University of Wisconsin-Madison, Madison, WI 53715, USA |
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Abstract: | Previous studies on the fourth inversion of the t complex, In17(4), suggest that loci near the center of this inversion have been subjected to segmental recombination during the past 1–2 million
years. We have used a combination of PCR-based restriction site (PBR) analysis and DNA sequencing to perform a high-resolution
analysis of a 2-million base pair (Mbp) segment in the middle of In17(4). We examined 21 restriction sites that are polymorphic between t haplotypes and their wild-type homologs, over nine distinct loci. In addition, we examined several other polymorphic sites
through DNA sequence analysis of two of these nine loci. We analyzed several haplotypes in this way, including the “complete”
t haplotypes t
w2
, t
0
, t
w32
, t
w71
, and t
w75
. We show that only t
w32
is a true “complete” t haplotype; the remaining four t haplotypes have segments of wild-type DNA ranging from less than 100 bp to 2 Mbp. The sizes of these wild-type DNA segments
are consistent with their being generated by gene-conversion events. The 2-Mbp segment is located in a region that may contain
the t-complex distorter gene Tcd2. One of the nine loci examined in this study is Fgd2, a gene that has been proposed to encode Tcd2. Sequencing and PBR data show that at least a portion of the Fgd2 gene has been converted to the wild-type within t
w71
and t
w75
mice. |
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Keywords: | |
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