Isolation and characterization of 24 microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid |
| |
Authors: | K F Rodrigues S V Kumar |
| |
Institution: | (1) Biotechnology Research Institute, Universiti Malaysia Sabah, Locked bag 2073, 88999 Kota Kinabalu, Sabah, Malaysia |
| |
Abstract: | This paper describes the characterization of 24 polymorphic microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid endemic to Sabah, Malaysia. A total of 36 clones containing microsatellite repeat motifs yielded
41 dinucleotide, 20 tetranucleotide and 30 cryptic simple repeat sequences. Thirty microsatellite loci were randomly selected
to characterize the population, of which 24 were found to be polymorphic. The expected heterozygosity ranged from 0.0813 to
0.7279 (mean 0.4533) and the observed heterozygosity ranged from 0 to 1 (mean 0.3800). The results indicated that there was
limited migration between the three subpopulations and a significant degree of genetic differentiation (FST = 0.5098) between populations implying that gene flow has been disrupted as a result of habitat fragmentation. The molecular
markers have a potential application in population management, CITES enforcement and the forensic detection of specimens in
international trade. |
| |
Keywords: | Paphiopedilum rothschildianum Microsatellite 5′ anchored PCR Heterozygosity CITES |
本文献已被 SpringerLink 等数据库收录! |
|