| 微生物发酵产生的番茄红素的分离纯化 |
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| 引用本文： | 苗振华,郑珩,劳兴珍,王聪,顾觉奋,强晓妍.微生物发酵产生的番茄红素的分离纯化[J].氨基酸和生物资源,2009,31(2):4-8. |
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| 作者姓名： | 苗振华 郑珩 劳兴珍 王聪 顾觉奋 强晓妍 |
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| 作者单位： | 中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京,210009;中国药科大学生命科学与技术学院,南京,210009 |
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| 基金项目： | 国家大学生创新实验计划 |
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| 摘 要： | 研究了三孢布拉霉(Blakeslea trispora)发酵产生的番茄红素的分离纯化方法。与植物源的番茄红素相比,真菌发酵产物中含有更多的油脂成分,且提取物中含有的一些同分异构体与产物性质类似而不易分离。实验采用溶剂抽提、大孔吸附树脂吸附法纯化以及结晶的方法可获得番茄红素晶体,纯度达70.9%,收率为64.6%。产品经重结晶后可获得纯度90%以上的番茄红素,经红外、核磁、质谱等证实与植物来源的番茄红素相一致。
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| 关 键 词： | 大孔吸附树脂 真菌 发酵法 番茄红素 分离纯化 |
Separation and Purification of Lycopene Obtained from Blakeslea trispora |
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| MIAO Zhen-hua,ZHENG Heng,LAO Xing-zhen,WANG Cong,GU Jue-fen,QIANG Xiao-yan.Separation and Purification of Lycopene Obtained from Blakeslea trispora[J].Amino Acids & Biotic Resources,2009,31(2):4-8. |
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| Authors: | MIAO Zhen-hua ZHENG Heng LAO Xing-zhen WANG Cong GU Jue-fen QIANG Xiao-yan |
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| Institution: | MIAO Zhen-hua,ZHENG Heng,LAO Xing-zhen,WANG Cong,GU Jue-fen,QIANG Xiao-yan(School of Life Science , Technology,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | Separation and purification of lycopene produced by Blakeslea trispora was studied.Compared with the method of tomato extraction,lycopene-producing fungi contained more lipids.It was hard to separate lycopene from other isomerides in the extract.The hyphostroma was extracted by petroleum ether and then purified by macroporous adsorption resin X-5 and then crystallized under-20 ℃.The purity of crystal was 70.9% and yield was 64.6%.After further recrystallisation the structure was confirmed by IR,1H-NMR and MS. |
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| Keywords: | macroporous adsorption resin fungi fermentation iycopene isolation and purification |
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