PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat |
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Authors: | P J Bebeli Z Zhou D J Somers J P Gustafson |
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Institution: | (1) Department of Plant Breeding and Biometry, Athens Agricultural University, Athens 11855, Greece, GR;(2) Department of Agronomy, Plant Genetics Research Unit, University of Missouri-Columbia, Columbia, MO 65211, USA, US;(3) Department of Agronomy and USDA Agriculture Research Service, Plant Genetics Research Unit, University of Missouri-Columbia, Columbia, MO 65211, USA, US |
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Abstract: | Four minisatellite core sequences were used as primers in a polymerase chain reaction (PCR) technique, known as the directed
amplification of minisatellite-region DNA (DAMD), to detect polymorphisms in three pairs of hexaploid/tetraploid wheat cultivars.
In each pair, the tetraploid cultivar (genomic formula AABB) was extracted from its corresponding hexaploid (genomic formula
AABBDD) parent. Reproducible profiles of the amplified products revealed characteristic bands that were present only in the
hexaploid wheats but not in their extracted tetraploids. Some polymorphisms were observed among the hexaploid cultivars. Twenty-three
DAMD-PCR amplified fragments were isolated and screened as molecular probes on the genomic DNA of wild wheat species, hexaploid
wheat and triticale cultivars. Subsequently, 8 of the fragments were cloned and sequenced. The DAMD-PCR clones revealed various
degrees of polymorphism among different wild and cultivated wheats. Two clones yielded individual-specific DNA fingerprinting
patterns which could be used for species differentiation and cultivar identification. The results demonstrated the use of
DAMD-PCR as a tool for the isolation of informative molecular probes for DNA fingerprinting in wheat cultivars and species.
Received: 13 May 1996/Accepted: 11 October 1996 |
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Keywords: | PCR Minisatellite DNA fingerprinting Wheat Triticale |
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