Stimulation of prostaglandin E2 synthesis in chondrocytes by a factor derived from activated macrophages |
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| Authors: | Joseph C. Colan Walter Pickett Karen English Arnold L. Oronsky S.S. Kerwar |
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| Affiliation: | Connective Tissue and Arthritis Research Unit Inflammation and Immunology Research Department Medical Research Division American Cyanamid Company Lederle Laboratories Pearl River, New York 10965, USA |
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| Abstract: | The serum-free spent medium of lipopolysaccharide-activated rabbit peritoneal macrophages contains a proteinaceous factor that stimulates the synthesis of PGE2 in rabbit articular chondrocytes. Synthesis of this factor by macrophages is inhibited by cycloheximide. Stimulation of PGE2 in chondrocytes is detected after a four-hour exposure to the macrophage factor and is completely abolished by the addition of either cycloheximide or indomethacin to the chondrocyte cultures. The macrophage derived factor has an apparent molecular weight of 30,000, is heat stable and not inactivated upon reductive alkylation or on treatment with phenylglyoxal. Activity is partially destroyed upon treatment with acid (pH 2.0) and upon trypsin treatment. |
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| Keywords: | All correspondence to this author at the above address. |
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