Molecular and chromosomal organization of two repetitive DNA sequences with intercalary locations in sugar beet and other Beta species |
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| Authors: | T Schmidt S Kubis A Katsiotis C Jung J S Heslop-Harrison |
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| Institution: | (1) Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany Tel: +49 431 8802581 Fax: +49 431 8802566 E-mail: Tschmidt@plantbreeding.uni-kiel.de, DE;(2) Karyobiology Group, John Innes Centre, Colney Lane, Norwich NR4 7UH, England, GB;(3) Laboratory of Genetics, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece, GR |
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| Abstract: | In a search for repetitive DNA sequences in the sugar beet genome, two sequences with repeat unit lengths of 143 and 434 bp
were isolated and characterized. The pSV family showed an unusual conservation of restriction sites reflecting homogenization
of the analyzed repeats. Members of the family are organized as tandem repeats as revealed by PCR and sequencing of dimeric
units. The pSV satellite occurs in large intercalary arrays which are present on all chromosome arms of sugar beet. The pSV
sequence family is present in different abundance in the sections Beta, Corollinae and Nanae but is not detectable by Southern hybridization in the section Procumbentes. The pDRV family is characterized by an interspersed genomic organization. The sequence is detectable in all sections of
the genus and is amplified in species of the section Beta but was also detected, although at lower abundance, in the remaining three sections. Fluorescent in situ hybridization has
shown that the pDRV sequence family is dispersed over all chromosomes of the sugar beet complement with some regions of clustering
and centromeric depletion.
Received: 18 March 1998 / Accepted: 31 March 1998 |
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| Keywords: | Satellite DNA Repetitive DNA Fluorescent in situ hybridization Beta vulgaris Dispersed repeats |
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