GAL4-responsive UAS-tau as a tool for studying the anatomy and development of the Drosophila central nervous system |
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Authors: | Kei Ito Heinz Sass Joachim Urban Alois Hofbauer Stephan Schneuwly |
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Institution: | German Primate Center, Department of Neurobiology, Kellnerweg 4, D-37077 G?ttingen, Germany, DE
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Abstract: | To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed
actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled
cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic
development.
Received: 28 February 1997 / Accepted: 10 May 1997 |
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Keywords: | : Brain Postembryonic development Metamorphosis Neurone Enhancer trap Drosophila melanogaster (Insecta) |
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