| Differentiation profile of brain tumor stem cells： a comparative study with neural stem cells |
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| 作者姓名： | Zhang QB Ji XY Huang Q Dong J Zhu YD Lan Q |
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| 作者单位： | Neurosurgical Department and Brain Tumor Research Laboratory, Second Affiliated Hospital of Suzhou University, Suzhou 215004,China |
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| 基金项目： | Acknowledgments The current research was supported by National Natural Sience Foundation of China（No.30371457 and 30400457）.We would express our thanks to the staff of Nerve Regeneration Key Laboratory of Jiangsu Province,Nantong University,who kindly helped to perform the lmmunofluorescent analysis with laser scanning confocal microscope. |
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| 摘 要： | Understanding of the differentiation profile of brain tumor stem cells （BTSCs）, the key ones among tumor cell population, through comparison with neural stem cells （NSCs） would lend insight into the origin of glioma and ultimately yield new approaches to fight this intractable disease. Here, we cultured and purified BTSCs from surgical glioma specimens and NSCs from human fetal brain tissue, and further analyzed their cellular biological behaviors, especially their differentiation property. As expected, NSCs differentiated into mature neural phenotypes. In the same differentiation condition, however, BTSCs exhibited distinguished differences. Morphologically, cells grew flattened and attached for the first week, but gradually aggregated and reformed floating tumor sphere thereafter. During the corresponding period, the expression rate of undifferentiated cell marker CD 133 and nestin in BTSCs kept decreasing, but 1 week later, they regained ascending tendency. Interestingly, the differentiated cell markers GFAP and β-tubulinlII showed an expression change inverse to that of undifferentiated cell markers. Taken together, BTSCs were revealed to possess a capacity to resist differentiation, which actually represents the malignant behaviors of glioma.
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| 关 键 词： | 脑癌干细胞 神经干细胞 分化 神经胶质瘤 |
| 收稿时间： | 2006-05-18 |
| 修稿时间： | 2006-05-18 |
Differentiation profile of brain tumor stem cells: a comparative study with neural stem cells |
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| Zhang QB,Ji XY,Huang Q,Dong J,Zhu YD,Lan Q.Differentiation profile of brain tumor stem cells: a comparative study with neural stem cells[J].Cell Research,2006,16(12):909-915. |
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| Authors: | Zhang Quan Bin Ji Xiao Yan Huang Qiang Dong Jun Zhu Yu De Lan Qing |
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| Institution: | Quan Bin Zhang~1 Xiao Yan Ji~1 Qiang Huang~1 Jun Dong~1 Yu De Zhu~1 Qing Lan~1 Neurosurgical Department and Brain Tumor Research Laboratory,Second Affiliated Hospital of Suzhou Universiry,Suzhou 215004,China |
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| Abstract: | Understanding of the differentiation profile of brain tumor stem cells (BTSCs), the key ones among tumor cell population, through comparison with neural stem cells (NSCs) would lend insight into the origin of glioma and ultimately yield new approaches to fight this intractable disease. Here, we cultured and purified BTSCs from surgical glioma specimens and NSCs from human fetal brain tissue, and further analyzed their cellular biological behaviors, especially their differentiation property. As expected, NSCs differentiated into mature neural phenotypes. In the same differentiation condition, however, BTSCs exhibited distinguished differences. Morphologically, cells grew flattened and attached for the first week, but gradually aggregated and reformed floating tumor sphere thereafter. During the corresponding period, the expression rate of undifferentiated cell marker CD133 and nestin in BTSCs kept decreasing, but 1 week later, they regained ascending tendency. Interestingly, the differentiated cell markers GFAP and beta-tubulinIII showed an expression change inverse to that of undifferentiated cell markers. Taken together, BTSCs were revealed to possess a capacity to resist differentiation, which actually represents the malignant behaviors of glioma. |
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| Keywords: | brain tumor stem cell neural stem cell differentiation |
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