Mg-activated double-stranded DNA cleavage by [(bpy)2(OH2)Ru---O---Ru(H2O) (bpy)2] |
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| Authors: | Neena Grover Suzanne A Ciftan H Holden Thorp |
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| Institution: | Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA |
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| Abstract: | The reaction of the title complex with DNA has been examined. Addition of (bpy)2(OH2)RuORu(OH2) (bpy)2]4+ to DNA leads to the reduction of the complex to Ru(bpy)2(OH2)22+, as indicated by absorption spectroscopy and cyclic voltammetry. The reaction is accelerated by Mg2+. The combined evidence points to a mechanism where the oxo-bridged dimer is hydrolyzed to a monomeric Ru(III) complex that is capable of oxidizing DNA to effect strand scission. Gel electrophoresis demonstrates nicking of supercoiled /gfX174 DNA by (bpy)2(OH2)RuORu(OH2) (bpy)2]4+, and double-stranded cleavage is observed in the presence of Mg2+. Linearization of the plasmid prior to treatment with the complex does not lead to further fragmentation, suggesting that supercoiling is required to realize double-stranded cleavage. |
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| Keywords: | Magnesium(II)-activated DNA cleavage Ruthenium complexes Oxo-bridged complexes Aquo complexes Bipyridine complexes |
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