Physiological and genomic properties of Thermus tenuipuniceus sp. nov., a novel slight reddish color member isolated from a terrestrial geothermal spring |
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Authors: | En-Min Zhou Wen-Dong Xian Jian-Yu Jiao Lan Liu Meng-Meng Li Yi-Ping Ding Yi-Rui Yin Jiao Zhao Salam Nimaichand Min Xiao Wen-Jun Li |
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Institution: | 1. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China;2. School of Resource Environment and Earth Science, Yunnan Institute of Geography, Yunnan University, Kunming 650091, People’s Republic of China;3. Shenzhen Key Laboratory of Bioenergy, Beijing Genomics Institute at Shenzhen (BGI-Shenzhen), Shenzhen 518083, People’s Republic of China |
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Abstract: | Two closely related, thermophilic bacteria, designated strains YIM 76954T and YIM 76947, were isolated from the Rehai Geothermal Field, Tengchong, Yunnan province, south-west China. Polyphasic approach and whole genome sequencing were used to determine the taxonomy status and genomic profiles of the novel strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates were closely related to Thermus scotoductus SE-1T (97.1% sequence similarity), and T. amyloliquefaciens YIM 77409T (96.6%). The strains could be differentiated from most recognized Thermus species by their whitish to slight reddish colony color, distinct DNA fingerprinting profiles and low ANI values. Cells stained Gram-negative, rod-shaped of diameter 0.2–0.5 μm and length 1.5–5.0 μm. Growth occurred at 50–75 °C, pH 6.0–9.0 and in the presence of up to 1.0% (w/v) NaCl concentration. Thiosulfate was found to enhance cell growth, besides improving the intensity of its colony color. Oxygen, nitrate, sulfur, and Fe(III) could be used as terminal electron acceptors for growth. MK-8 was the major respiratory menaquinone. Major fatty acids were iso-C17:0, iso-C15:0, anteiso-C17:0, and anteiso-C15:0. The genome size was 2.26 Mbp with 65.5% average GC content. A total of 2374 genes was predicted, comprising 2322 protein-coding and 52 RNA genes. On the basis of the polyphasic evidence presented, it is proposed that strain YIM 76954T represents a novel species of the genus Thermus, for which the name Thermus tenuipuniceus sp. nov. is proposed. The type strain is YIM 76954T (=JCM 30350T = KCTC 4677T). |
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Keywords: | ANI average nucleotide identity dDDH digital DNA–DNA hybridization Polyphasic taxonomy Genome sequencing Tengchong hot springs DNA fingerprinting |
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