| 稻瘟病菌(Magnaporthe grisea)诱导的水稻早期反应基因ER1的5' 端cDNA片段克隆及序列分析 |
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| 引用本文： | 周建明,朱群,白永延. 稻瘟病菌(Magnaporthe grisea)诱导的水稻早期反应基因ER1的5' 端cDNA片段克隆及序列分析[J]. 植物生理与分子生物学学报, 1999, 25(4) |
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| 作者姓名： | 周建明 朱群 白永延 |
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| 作者单位： | 中国科学院上海植物生理研究所!上海200032，SalkInstituteforBiologicalStudies!CA92037，USA，中国科学院上海植物生理研究所!上海200032 |
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| 基金项目： | 中国科学院上海植物生理研究所植物分子遗传国家重点实验室资助 |
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| 摘 要： | 研究高等生物基因表达与调控的一个重要方面是分离基因的编码区及其上游的调控序列（ＤｅＶｅｅｒ等１９９７），这需要获得一个基因的ｃＤＮＡ全长及从植物基因组获取全基因。在前文（周建明等１９９９）中曾经分离了稻瘟病菌侵染诱导的水稻早期反应基因ＥＲ１的ｃＤＮＡ片段，但是运用ｍＲＮＡ差异显示技术分离的ｃＤＮＡ片段往往只有近ｍＲＮＡ３’端的一部分，难以反映基因的结构及功能特点，因此，必须进一步分离其５’端的部分才有可能比较全面地了解此基因的特点。ＲＡＣＥ（ｒａｐｉｄａｍｐｌｉｆｉｃａｔｉｏｎｏｆｃＤＮＡｅｎ…
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| 关 键 词： | 水稻 稻瘟病菌 ER1基因 5’cDNA片段 5’RACE |
Cloning and Sequencing of 5' end cDNA Fragment of Early Response Gene(ER1) of Rice Induced by the Blast Fungus Magnaporthe grisea |
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| ZHOU Jian Ming ,ZHU Qun and BAI Yong Yan. Cloning and Sequencing of 5' end cDNA Fragment of Early Response Gene(ER1) of Rice Induced by the Blast Fungus Magnaporthe grisea[J]. Journal Of Plant Physiology and Molecular Biology, 1999, 25(4) |
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| Authors: | ZHOU Jian Ming ZHU Qun BAI Yong Yan |
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| Affiliation: | ZHOU Jian Ming 1,ZHU Qun 2 and BAI Yong Yan 1 |
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| Abstract: | The 5' end cDNA fragment(ER1') of early responsive gene(ER1) of rice(Oryza sativa L. subsp. japonica No.4) induced by blast fungus M. grisea has been isolated and sequenced (606 bp) by 5'RACE technique(Fig. 2). DNA sequence analysis showed that ER1' (606 bp) can encode 134 amino acids and there are 309 bp nucleotides located at the 3' end of the untranslated region of mRNA of ER1 gene(Fig. 3). The 89 bp overlapping fragment was found between the 5' end of ER1 cDNA fragment and the 3' end of ER1'(Fig. 3). The partial identities of amino acid sequence deduced from ER1' with an unknown protein(gene accession number AC002409) of Arabidopsis thaliana was 82%(Fig. 4). |
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| Keywords: | rice Magnaporthe grisea ER1 gene 5' end cDNA(ER1') 5'RACE |
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