Characterisation ofEscherichia coli isolated from raw milk cheeses |
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Authors: | Miriam Zago Barbara Bonvini Antonio M Martín Platero Germano Mucchetti Domenico Carminati Giorgio Giraffa |
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Institution: | 1. C.R.A.-Istituto Sperimentale Lattiero-Caseario, Via Lombardo 11, 26900, Lodi, Italy 2. Department of Microbiology, University of Granada, Avda. Fuentenueva s/n., 18071, Granada, Spain 3. Department of Industrial Engineering, University of Parma, Parco Area delle Scienze 181/A, 43100, Parma, Italy
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Abstract: | The aim of this work was: (i) to verify the level ofEscherichia coli in Pannerone and Valtrompia Formaggella, two artisanal Italian raw-milk cheeses ripened for less than 60 days; (ii) to phenotypically and genotipycally type theE. coli isolates; (iii) to detect the presence ofE. coli O157:H7 and of intestinal enteropathogenicE. coli by PCR. The levels ofE. coli in the cheeses ranged from 3.89 to 8.47 log CFU g?1. NoE. coli O157:H7 was detected in 25 g of cheese. The 76E. coli strains (68 cheese isolates and 8 reference strains) were widely diverse, since a high number of both PCR fingerprinting profiles and PhenePlate® phenotypes were shown. Within the 68 cheese isolates, no toxin production and virulence-associated genes were shown by multiplex PCR. Non-pathogenicE. coli were isolated at high levels in raw-milk cheeses, where they may contribute to the development of desirable characteristics of some of these products, e.g. Pannerone. |
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