| 农杆菌介导的玉米大斑病菌转化 |
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| 引用本文： | 周永力 TANAKA Chihiro TSUDA Mitusya.农杆菌介导的玉米大斑病菌转化[J].菌物学报,2003,22(3):374-379. |
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| 作者姓名： | 周永力 TANAKA Chihiro TSUDA Mitusya |
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| 作者单位： | 1. 中国农业科学院作物育种栽培研究所农业部遗传育种重点实验室,北京,100081
2. 京都大学环境微生物实验室,日本,京都,606-8502 |
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| 摘 要： | 采用玉米大斑病菌Exserohilum turcicum的无性菌丝作受体,建立了农杆菌Agrobacterium tumefaciens介导的转化方法。采用的载体为 pUR5750, 携有来源于质粒pAN71的潮霉素抗性选择标记。PCR分析表明T-DNA 插入到E. turcicum基因组中。
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| 关 键 词： | T-DNA 插入 潮霉素B 菌丝 |
GENETIC TRANSFORMATION OF EXSEROHILUM TURCICUM MEDIATED BY AGROBACTERIUM TUMEFACIENS |
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| Authors: | TANAKA Chihiro TSUDA Mitusya |
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| Abstract: | A transformation technique for Exserohilum turcicum mediated by Agrobacterium tumefaciens using vegetative mycelium as recipient was developed and procedure for the enhancement of transformation efficiency was optimized. The binary vector used in transformation was pUR5750, which contains selection marker gene conferring hygromycin-resistance under control of the Aspergillus nidulans gpd promoter and trpC terminator between the left border and right border repeats of the binary vector pBIN19. PCR analysis showed that T-DNA was integrated into the genome of E. turcicum. |
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| Keywords: | T-DNA integration hygromycin B mycelium |
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