Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49 |
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Authors: | Bing-Feng Liu Nan-Qi RenDe-Feng Xing Jie DingGuo-Xiang Zheng Wan-Qian GuoJi-Fei Xu Guo-Jun Xie |
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Institution: | State Key Lab of Urban Water Resource and Environment (SKLUWRE), School of Municipal & Environmental Engineering, Harbin Institute of Technology, No. 202 Haihe Road, 2nd Campus of HIT Box 2614, Harbin 150090, China |
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Abstract: | In order to increase the hydrogen yield from glucose, hydrogen production by immobilized Rhodopseudomonas faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria Ethanoligenens harbinense B49 was investigated. The soluble metabolites from dark-fermentation mainly were ethanol and acetate, which could be further utilized for photo-hydrogen production. Hydrogen production by B49 was noticeably affected by the glucose and phosphate buffer concentration. The maximum hydrogen yield (1.83 mol H2/mol glucose) was obtained at 9 g/l glucose. In addition, we found that the ratio of acetate/ethanol (A/E) increased with increasing phosphate buffer concentration, which is favorable to further photo-hydrogen production. The total hydrogen yield during dark- and photo-fermentation reached its maximum value (6.32 mol H2/mol glucose) using 9 g/l glucose, 30 mmol/l phosphate buffers and immobilized R. faecalis RLD-53. Results demonstrated that the combination of dark- and photo- fermentation was an effective and efficient process to improve hydrogen yield from a single substrate. |
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Keywords: | Bio-hydrogen Ethanoligenens harbinense Rhodopseudomonas faecalis Soluble metabolites Phosphate buffer |
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