Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters |
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Authors: | Shujie Cheng Peizhou Yang Liqiong Guo Junfang Lin Nannan Lou |
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Institution: | 1. Department of Bioengineering, College of Food Science, South China Agricultural University, 482 Wushan Street, Tianhe, Guangzhou 510640, China;2. Institute of Biomass Research, South China Agricultural University, Guangzhou 510640, China;3. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China |
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Abstract: | Multi-functional cellulase gene mfc was expressed in Coprinus cinereus under naturally non-inductive conditions using three heterologous promoters. Endo-β-1,4-glucanase expression was achieved in solid and liquid media with promoter sequences from the Lentinula edodesgpd gene, the Flammulina velutipes gpd gene and the Volvariella volvaceagpd gene. As measured by enzyme activity in liquid cultures, a 613-bp gpd promoter fragment from L. edodes was most efficient, followed by a 752-bp gpd fragment from F. velutipes. The V. volvacea gpd promoter sequence was less active, in comparison. Irrespective of the promoter used, enzymatic activities increase 34-fold for highly active transformants and 29-fold for less active one by using cellulase-inducing medium. The highest activities of endo-β-1,4-glucanase (34.234 U/ml) and endo-β-1,4-xylanase (263.695 U/ml) were reached by using the L. edodesgpd promoter. |
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Keywords: | Multi-functional cellulase Endo-β-1 4-glucanase Endo-β-1 4-xylanase Coprinus cinereus Basidiomycete promoters |
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