A refinery sludge deposition site: presence of nahH and alkJ genes and crude oil biodegradation ability of bacterial isolates |
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Authors: | Nikolaos Arvanitis Efstathios A Katsifas Kalliopi I Chalkou Christos Meintanis Amalia D Karagouni |
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Institution: | (1) Faculty of Biology, Department of Botany, Microbiology Group, National and Kapodistrian University of Athens, 15781 Athens, Greece |
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Abstract: | 204 bacterial isolates from four Greek refinery sludge deposition sites were investigated for the presence of nahH and alkJ genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by PCR and DNA hybridisation.
Members of Pseudomonas, Acinetobacter, Bacillus, Rhodococcus and Arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahH and alkJ genes were present in the 73% of the isolates. Consortia of bacterial isolates that were used for biodegradation of aliphatic
and aromatic hydrocarbons in crude oil using liquid cultures exhibited rates from 35% to 48% within 10 days of incubation.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Bacteria Biodegradation Genes for hydrocarbon dehydration (nahH and alkJ) Hydrocarbons |
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