Midgut infection by tomato spotted wilt virus and vector incompetence of Frankliniella tritici |
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| Authors: | F M de Assis Filho J Stavisky S R Reitz C M Deom J L Sherwood |
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| Institution: | Department of Plant Pathology, The University of Georgia, Athens, GA;;NYS Integrated Pest Management Program, NYSAES, Geneva, NY;;USDA-ARS, Center for Biological Control, Tallahassee, FL, USA |
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| Abstract: | Abstract: The mechanism leading to vector competence of thrips species to transmit tomato spotted wilt virus (TSWV) is not well characterized. We investigated the interaction of TSWV and the non-vector species Frankliniella tritici . A monoclonal antibody to the non-structural protein (NSs) of the TSWV was used to detect TSWV replication within the thrips by immunofluorescence microscopy and enzyme-linked immonosorbent assay (ELISA). TSWV was acquired by F. tritici , replicated and moved within the alimentary canal of F. tritici similar to a known vector of TSWV, Frankliniella occidentalis . However, virus was not found in the salivary glands of F. tritici , which is a prerequisite to virus transmission. Thus, movement to the salivary glands may determine vector incompetence of F. tritici . |
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| Keywords: | ELISA fluorescence microscopy thrips transmission TSWV |
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