Bioconversion of a ganoderic acid 3-hydroxy-lanosta-8,24-dien-26-oic acid by a crude enzyme from Ganoderma lucidum |
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| Institution: | 1. Engineering Research Center of Bio-Process of Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, PR China;2. Hefei Preschool Education College, Hefei, 230013, PR China;1. Tecnología Enzimática para Bioprocesos, Departamento de Ingeniería de Alimentos, Universidad de La Serena, Raúl Bitran N° 1305, La Serena, Chile;2. Laboratorio de Bromatología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco N° 917, San Luis, Argentina;3. Instituto de Física Aplicada (INFAP) – CCT - San Luis - CONICET, Avenida Ejército de los Andes N° 950, Bloque II, 2° Piso, 5700, San Luis, San Luis, Argentina;4. Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Avenida Brasil N° 2085, Valparaíso, Chile;5. Laboratorio de Péptidos, Núcleo de Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Avenida Universidad N° 330, Curauma, Valparaíso, Chile;6. Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Facultad de Ingeniería y Química, Universidad Católica Argentina, Av. Pellegrini 3314, Rosario, Santa Fé, Argentina;7. Centro de Investigación de Proteínas Vegetal (CIProVe), Universidad Nacional de la Plata, Calle 47 y 115, La Plata, Argentina;8. Instituto de Investigación Multidisciplinario en Ciencia y Tecnología, Universidad de La Serena, Raúl Bitran N° 1305, La Serena, Chile;1. College of Environmental Science and Engineering, Taiyuan University of Technology, Shanxi 030024, China;2. Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Shanxi 030024, China;1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China;2. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, China;3. Zhoushan Institute for Food and Drug Control, Zhoushan, 316000, China;1. Radiation Utilization and Facilities Management Division, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 56212, Republic of Korea;2. Department of Bioactive Material Sciences, Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Jeonbuk National University, Jeonju 54896, Republic of Korea;3. Bacteria Research Team, Nakdonggang National Institute of Biological Resources (NNIBR), Sangju 37242, Republic of Korea;4. Radiation Research Division, Korea Atomic Energy Research Institute, 29 Geumgu-gil, Jeongeup 56212, Republic of Korea;5. Department of Radiation Science and Technology, University of Science and Technology, Daejeon 34113, Republic of Korea |
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| Abstract: | Ganoderic acids (GAs) are oxygenated lanostane-type triterpenoids from the traditional medicinal mushroom Ganoderma lucidum and of significant biological activities. Although a ganoderic acid 3-hydroxy-lanosta-8,24-dien-26-oic acid (HLDOA) was found to be biosynthesized from lanosterol, further post-modification of HLDOA is yet unclear. In this work, by using HLDOA as the substrate and a crude enzyme from G. lucidum as the biocatalyst, we observed a new peak in liquid chromatography from the reaction system. The product was purified and identified to be 3-oxo-lanosta-8,24-dien-26-oic acid (OLDOA), which may be converted from HLDOA by a putative dehydrogenase of G. lucidum. The work is useful to future manufacture of GAs as well as their biosynthetic pathway elucidation. |
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| Keywords: | Basidiomycete mushroom triterpenoid ganoderic acid bioconversion |
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