Screening of lipases for production of novel structured lipids from single cell oils |
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| Institution: | 1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, PR China;2. Food Science and Technology Department, Faculty of Environmental Agricultural Science, Arish University, North Sinai 45516, Egypt;3. Department of Clinical Nutrition, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia;4. Department of Food Science, Faculty of Agriculture, Zagazig University, 44511 Zagazig, Egypt;1. Department of Engineering, Aarhus University, Gustav WiedsVej 10, 8000 Aarhus C, Denmark;2. College of Life Science, Fujian Normal University, No.1, Keji Road, Minhou, Fuzhou 350117, China;3. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, No.1, Keji Road, Minhou, Fuzhou 350117, China;1. College of Life Science, Fujian Normal University, Fuzhou 350117, China;2. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, Fuzhou 350117, China;3. Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;1. National Engineering Research Centre for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, PR China;2. Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia;3. Wageningen University & Research, Wageningen, the Netherlands;4. Department of Food Engineering, Faculty of Agriculture, Ataturk University, Erzurum, 25240, Turkey;1. Departamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Brazil;2. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;3. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, Ceará, Brazil;4. Departamento de Engenharia Química, Universidade Federal do Rio Grande do Norte, Natal, Brazil;5. Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Nilópolis, Brazil;6. Programa de Engenharia Química, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;7. Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;8. Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, 28049, Madrid, Spain;9. Center of Excellence in Bionanoscience Research, External Scientific Advisory Academic, King Abdulaziz University, Jeddah, 21589, Saudi Arabia;1. College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China;2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China;3. Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USA |
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| Abstract: | Lipids enriched in polyunsaturated fatty acids are very susceptible to oxidation, causing the formation of potentially harmful oxidized products. Hence, it is critical to keep the temperature as low as possible during reaction and storage. In this study, five commercial immobilized lipases were evaluated for their capability to produce novel structured lipids (SLs) enriched with medium-chain fatty acids (MCFAs) through acidolysis of single cell oil (SCO) with capric acid. Among the examined lipases, NS40086 and Lipozyme RM IM showed the highest incorporation degree. The acidolysis reactions resulted in an obvious variation in the fatty acids composition as well as their positional distribution. The obtained SLs contained (33.58 %–34.09 %) capric acid at sn-1, 3 positions with increasing the content of arachidonic acid at the sn-2 position up to (49.82 %–50.25 %). The NS40086 lipase displayed 1, 3 regiospecificity towards the TAG of SCO. The acidolysis reactions using NS40086 lipase resulted in a generation of 23 TAG molecular species containing capric acid. Moreover, the NS40086 lipase was more active than Lipozyme RM IM at relatively low temperatures (35 °C and 40 °C), which could be used effectively as a promising biocatalyst in lipid synthesis. |
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| Keywords: | Lipase-catalyzed acidolysis Single cell oil Structured lipids NS40086 lipase Capric acid |
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