Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide |
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| Institution: | 1. Department of Biotechnology, Graduate School, Korea University, Seoul 02841, South Korea;2. Clean Energy Research Center, Korea Institute of Science and Technology, Seoul 02792, South Korea;3. Division of Energy and Chemical Engineering, University of Science and Technology, Daejeon 34113, South Korea;1. Department of Biomedical Engineering, University of Alabama at Birmingham (UAB), 1825 University Blvd., Birmingham, AL 35233, USA;2. Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA;3. Department of Medicine, University of Alabama at Birmingham, 703 19th Street South, Birmingham, AL 35294, USA;4. Departments of Pediatric Dentistry and Microbiology, University of Alabama at Birmingham, 1919 7th Avenue South, Birmingham, AL 35233, USA;1. Department of Biological and Environmental Engineering, Cornell University, Riley-Robb Hall, Ithaca, NY 14853, United States;2. LanzaTech, 8045 Lamon Avenue, Suite 400, Skokie, IL 60077, United States;1. Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, United States;2. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, United States;1. LEQUIA, Institute of the Environment, University of Girona, Campus Montilivi, E-17071 Girona, Catalonia, Spain;2. Group of Molecular Microbial Ecology, Institute of Aquatic Ecology (IEA), University of Girona, Campus Montilivi, E-17071 Girona, Catalonia, Spain;1. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Shanghai Research and Development Center of Industrial Biotechnology, Shanghai 201201, China;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation. |
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| Keywords: | Metabolomics Extraction solvent Optimization Carbon monoxide |
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