花脸香蘑菌丝体提取物的体外抗氧化活性

分别测定花脸香蘑Lepista sordida发酵菌丝体不同浓度乙醇溶剂提取液在4种抗氧化模型中的抗氧化作用。结果表明, 各种菌丝体提取液对二苯基苦味酰基苯肼自由基(·DPPH)和羟自由基(·OH)均有显著的清除作用, 其清除作用随着乙醇溶剂浓度的提高而降低: 在3 g/L菌丝生药浓度时, 去离子水提取物对DPPH自由基和羟自由基的清除率分别为90.55%和81.9%; 在6 g/L菌丝生药浓度时, 50%乙醇和70%乙醇提取物对DPPH自由基的清除率分别为53.7%和45.2%, 对羟自由基的清除率分别为79.4%和78.4%。各菌丝提取物也具有极强的抗脂质过氧化能力, 在5 g/L菌丝生药浓度64 h内, 水提物、50%乙醇和70%乙醇提取物对亚油酸过氧化物的抑制率分别为100%、96%和89.2%。在一定浓度下, 各提取液对邻苯三酚自氧化也均有抑制作用。

The antioxidant activities of mycelial extracts of Lepista sordida in vitro

The antioxidant activities of fermented mycelial extracts with different concentrations of ethanol of Lepista sordida were individually determined in four test systems. The results showed that all the mycelial extracts had obvious effects on scavenging 1,1-diphenyl-2-picrylhydrazyl radical (·DPPH) and hydroxyl radical; and with the concentration of ethanol increasing, the antioxidant activities declined: the extract with deionized water at the concentration of 3 g/L mycelium, DPPH radical scavenging activity and hydroxyl radical scavenging activities were 90.55% and 81.9% respectively. At a concentration of 6 g/L mycelium in 50% and 70% ethanol extracts, the DPPH radical scavenging activities were 53.7% and 45.2%, and the hydroxyl radical scavenging activities were 79.4% and 78.4%. The extracts could also significantly inhibited the peroxidation of linoleic acid, within 64 hours at the concentration of 5 g/L, the inhibition ratios of the extracts with water, 50% and 70% ethanol were 100%, 96% and 89.2% respectively. The extracts possessed slightly restraining effects on pyrogallol autooxidation.