Substoichiometric shifting in the fertility reversion of cytoplasmic male sterile pearl millet |
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Authors: | X Feng A P Kaur S A Mackenzie I M Dweikat |
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Institution: | (1) Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583-0915, USA;(2) Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68583-0915, USA;(3) Present address: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD 21201, USA |
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Abstract: | Cytoplasmic male sterility (CMS) represents an important agricultural trait in pearl millet Pennisetum glaucum (L.) R. Br.] with a value to the seed industry in facilitating economical hybrid seed production. Among the CMS systems available
in millet, the A1 source is the most commonly used for hybrid production, but it can undergo low frequency reversion to fertility.
Plant mitochondrial genomes are highly recombinogenic, becoming unstable and prone to ectopic recombination under conditions
of tissue culture, somatic hybridization, or interspecific crossing. Similarly, CMS systems prone to spontaneous fertility
reversion experience sporadic mitochondrial genome instability. We compared mitochondrial genome configurations between the
male-sterile A1 line and fertile revertants of pearl millet to develop a model for millet mitochondrial genome reorganization
upon reversion. Relative copy number of a subgenomic molecule containing the CoxI-1-2 junction region, a component of the recombination process for reversion, is amplified tenfold following reversion, relative
to the CMS A1 line. We propose that increased copy number of this molecule in a small number of cells or at low frequency
triggers a recombination cascade, likely during reproductive development. The proposed recombination process initiates with
ectopic recombination through a 7-bp repeat to produce a novel CoxI-3-2 junction molecule and an unstable recombination intermediate. Subsequent intra-molecular recombination stabilizes the intermediate
to form a new copy of CoxI accompanied by a deletion. This study furthers the argument that substoichiometric shifting within the plant mitochondrial
genome plays an important role in the evolution of the mitochondrial genome and plant reproductive dynamics. |
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