NMR exposure sensitizes tumor cells to apoptosis |
| |
Authors: | L Ghibelli C Cerella S Cordisco G Clavarino S Marazzi M De Nicola S Nuccitelli M D'Alessio A Magrini A Bergamaschi V Guerrisi L M Porfiri |
| |
Institution: | (1) Dipartimento di Biologia, Universita' di Roma Tor Vergata, Rome, Italy;(2) Dipartimento di Radiologia, Universita' di Roma La Sapienza, Roma, Italy;(3) Cattedra Medicina del Lavoro, Universita' di Roma Tor Vergata, Roma, Italy;(4) Dipartimento di Biologia, Universita' di Roma “Tor Vergata”, Via della Ricerca Scientifica, 1, Rome, 00133, Italy |
| |
Abstract: | NMR technology has dramatically contributed to the revolution of image diagnostic. NMR apparatuses use combinations of microwaves
over a homogeneous strong (1 Tesla) static magnetic field. We had previously shown that low intensity (0.3–66 mT) static magnetic
fields deeply affect apoptosis in a Ca2+ dependent fashion (Fanelli et al., 1999 FASEBJ., 13;95–102). The rationale of the present study is to examine whether exposure to the static magnetic fields
of NMR can affect apoptosis induced on reporter tumor cells of haematopoietic origin. The impressive result was the strong
increase (1.8–2.5 fold) of damage-induced apoptosis by NMR. This potentiation is due to cytosolic Ca2+ overload consequent to NMR-promoted Ca2+ influx, since it is prevented by intracellular (BAPTA-AM) and extracellular (EGTA) Ca2+ chelation or by inhibition of plasma membrane L-type Ca2+ channels. Three-days follow up of treated cultures shows that NMR decrease long term cell survival, thus increasing the efficiency
of cytocidal treatments. Importantly, mononuclear white blood cells are not sensitised to apoptosis by NMR, showing that NMR
may increase the differential cytotoxicity of antitumor drugs on tumor vs normal cells. This strong, differential potentiating
effect of NMR on tumor cell apoptosis may have important implications, being in fact a possible adjuvant for antitumor therapies. |
| |
Keywords: | apoptosis blood cells Ca2+ influx etoposide NMR static magnetic field tumor cells |
本文献已被 PubMed SpringerLink 等数据库收录! |
|