| 苯甲醛高耐受性酵母菌的选育 |
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| 引用本文： | 梁新乐. 苯甲醛高耐受性酵母菌的选育[J]. 生物学杂志, 2003, 20(1): 30-31,39 |
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| 作者姓名： | 梁新乐 |
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| 作者单位： | 浙江杭州商学院生物工程系,杭州,310035 |
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| 摘 要： | 介绍一种经过长期诱导、驯化作用来选育耐性菌株的方法。通过固定化细胞的间歇补料培养方式和长期诱导、驯化后，筛选出8株具有较高苯甲醛耐受性的酿酒酵母菌株，其中菌株Sbht-35-23对苯甲醛的耐受性达到0．9％，并保持较高的稳定活性。采用这些具有较高耐性的酿酒酵母菌株生物合成L-苯基乙酰基甲醇(L-PAC)，将有利于提高转化率。
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| 关 键 词： | 苯甲醛高耐受性酵母菌 诱导驯化 选育 耐性菌株 |
| 文章编号： | 1008-9632(2003)01-0030-02 |
Screening of Saccharomyces cerevisiae strains with high tolerance to benzaldehyde for L-PAC production |
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| LIANG Xin-le. Screening of Saccharomyces cerevisiae strains with high tolerance to benzaldehyde for L-PAC production[J]. Journal of Biology, 2003, 20(1): 30-31,39 |
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| Authors: | LIANG Xin-le |
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| Abstract: | Using immobilized Saccharomyces cereviesiae cells repeatedly inducted by benzaldehyde with reactivation by fed-cultivation,eight strains with high tolerance to benzaldehyde were obtained.The result showed that strain Sbht-35-23 was high tolerance to benzaldehyde and a high stability among all obtained strains.Application of such strains would benefit to the biotransformation of L-phenylacetylcarbinol by benzaldehyde and pyruvate. |
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| Keywords: | benzaldehyde saccharomyces cereviesiae l-phenylacetylcarbinol tolerance |
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